|
KMID : 0378019740170111579
|
|
New Medical Journal
1974 Volume.17 No. 11 p.1579 ~ p.1585
|
|
|
Histochernical Study on the Effect of NAD on the Acid Mucopolysaccharide and Lipid of the Scorbutic Bone Tissue
|
|
|
|
|
Abstract
|
|
|
It is known that TAD, which operate as hydrogen and electron transfer agents by virture of reve￢rsible oxidation and reduction, play a vital role in metabolism.
The author observed the effect of NAD and NAD in combination with biotin on the acid mucopolysa￢ccharide and lipid in the scorbutic tibia of the guinea pig. Experimental guinea pigs were fed vitamin C-deficient diet modified by Anderson-Smith during experiment. The ´tibia was decalcified in `the ED￢TA solution (pH 7.4) and embedded in paraffin by an electric vacuum pump.
The histochemical and histological techniques employed were alcian blue-PAS modification method, alcian blue stain, aceton-Sudan black B method, performic acid-Schiff method, peracetic acid-Halmi method, von Kossa-hematoxylin stain and hematoxylin-eosin stain.
The results were as follows:
1) In the cartilage matrix of the scorbutic tibia, acid mucopolysaccharide reactions by the alcian blue were decreased than of the normal tibia, but these reactions were partly increased by NAD administration.
2) NAD led in the cartilage matrix of the scorbutic tibia to a increase of the lipid reaction by aceton-Sudan black B method, and, in the NAD-biotin administered groups, bone marrow showed positive reaction of the performic acid-Schiff method for lipid.
3) It seemed that NAD had the activity recovering the disturbed cartilage matrix formation in the scorbutic tibia.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|